Polyadenylation of a functional mRNA controls gene expression in Escherichia coli. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nucleic Acids Research Année : 2007

Polyadenylation of a functional mRNA controls gene expression in Escherichia coli.

Résumé

Although usually implicated in the stabilization of mRNAs in eukaryotes, polyadenylation was initially shown to destabilize RNA in bacteria. All the data are consistent with polyadenylation being part of a quality control process targeting folded RNA fragments and non-functional RNA molecules to degradation. We report here an example in Escherichia coli, where polyadenylation directly controls the level of expression of a gene by modulating the stability of a functional transcript. Inactivation of poly(A)polymerase I causes overexpression of glucosamine-6-phosphate synthase (GlmS) and both the accumulation and stabilization of the glmS transcript. Moreover, we show that the glmS mRNA results from the processing of the glmU-glmS cotranscript by RNase E. Interestingly, the glmU-glmS cotranscript and the mRNA fragment encoding GlmU only slightly accumulated in the absence of poly(A)polymerase, suggesting that the endonucleolytically generated glmS mRNA harbouring a 5' monophosphate and a 3' stable hairpin is highly susceptible to poly(A)-dependent degradation.

Dates et versions

hal-00196100 , version 1 (12-12-2007)

Identifiants

Citer

Géraldine Joanny, Jacques Le Derout, Dominique Bréchemier-Baey, Valérie Labas, Joelle Vinh, et al.. Polyadenylation of a functional mRNA controls gene expression in Escherichia coli.. Nucleic Acids Research, 2007, 35 (8), pp.2494-502. ⟨10.1093/nar/gkm120⟩. ⟨hal-00196100⟩
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