Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression.
André-Patrick Arrigo
(1)
,
Wance J J Firdaus
(1)
,
Grégory Mellier
,
Maryline Moulin
(1)
,
Catherine Paul
(1)
,
Chantal Diaz-Latoud
(1)
,
Carole Kretz-Remy
(1)
Grégory Mellier
- Fonction : Auteur
Maryline Moulin
- Fonction : Auteur
- PersonId : 740405
- IdHAL : maryline-moulin
- ORCID : 0000-0002-1851-8727
- IdRef : 124605680
Résumé
There is currently great interest in the development of methods to analyze intracellular redox state and the cellular damages generated by oxidative stress. General methods for analyzing reactive oxygen species and glutathione level are presented together with more recently developed protocols to analyze the consequences of oxidative stress on the oxidation of macromolecules. Finally, techniques to study modalities of constitutive expression of Hsp27 in mammalian cells are considered as well as methods used to determine the protective activity of this small heat shock protein against the deleterious effects induced by oxidative stress.
Domaines
Biologie cellulaireFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Cytotoxic effects induced by oxidative stress in cultured mammalian cells and protection provided by Hsp27 expression.
|
Résumé |
en
There is currently great interest in the development of methods to analyze intracellular redox state and the cellular damages generated by oxidative stress. General methods for analyzing reactive oxygen species and glutathione level are presented together with more recently developed protocols to analyze the consequences of oxidative stress on the oxidation of macromolecules. Finally, techniques to study modalities of constitutive expression of Hsp27 in mammalian cells are considered as well as methods used to determine the protective activity of this small heat shock protein against the deleterious effects induced by oxidative stress.
|
Auteur(s) |
André-Patrick Arrigo
1
, Wance J J Firdaus
1
, Grégory Mellier
, Maryline Moulin
1
, Catherine Paul
1
, Chantal Diaz-Latoud
1
, Carole Kretz-Remy
1
1
CGPhiMC -
Centre de génétique et de physiologie moléculaire et cellulaire
( 711 )
- bat. 741 43 Bvd du 11 Novembre 1918 69622 VILLEURBANNE CEDEX
- France
|
Nom de la revue |
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Audience |
Non spécifiée
|
Date de publication |
2005-02
|
Date de publication électronique |
2004-12-19
|
Volume |
35
|
Numéro |
2
|
Page/Identifiant |
126-38
|
Domaine(s) |
|
Mots-clés (Mesh) |
|
DOI | 10.1016/j.ymeth.2004.08.003 |
Pubmed Id | 15649839 |
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