Overexpression of the cholesterol-binding protein MLN64 induces liver damage in the mouse. - Archive ouverte HAL Access content directly
Journal Articles World Journal of Gastroenterology Year : 2007

Overexpression of the cholesterol-binding protein MLN64 induces liver damage in the mouse.

Juan-Enrique Tichauer
  • Function : Author
Maria-Gabriela Morales
  • Function : Author
Ludwig Amigo
  • Function : Author
Leopoldo Galdames
  • Function : Author
Andres Klein
  • Function : Author
Veronica Quinones
  • Function : Author
Carla Ferrada
  • Function : Author
Alejandra-R Alvarez
  • Function : Author
Juan-Francisco Miquel
  • Function : Author
Attilio Rigotti
  • Function : Author
Silvana Zanlungo
  • Function : Author

Abstract

AIM: To examine the in vivo phenotype associated with hepatic metastatic lymph node 64 (MLN64) over-expression. METHODS: Recombinant-adenovirus-mediated MLN64 gene transfer was used to overexpress MLN64 in the livers of C57BL/6 mice. We measured the effects of MLN64 overexpression on hepatic cholesterol content, bile flow, biliary lipid secretion and apoptosis markers. For in vitro studies cultured CHO cells with transient MLN64 overexpression were utilized and apoptosis by TUNEL assay was measured. RESULTS: Livers from Ad.MLN64-infected mice exhibited early onset of liver damage and apoptosis. This response correlated with increases in liver cholesterol content and biliary bile acid concentration, and impaired bile flow. We investigated whether liver MLN64 expression could be modulated in a murine model of hepatic injury. We found increased hepatic MLN64 mRNA and protein levels in mice with chenodeoxycholic acid-induced liver damage. In addition, cultured CHO cells with transient MLN64 overexpression showed increased apoptosis. CONCLUSION: In summary, hepatic MLN64 over-expression induced damage and apoptosis in murine livers and altered cholesterol metabolism. Further studies are required to elucidate the relevance of these findings under physiologic and disease conditions.
No file

Dates and versions

hal-00189174 , version 1 (20-11-2007)

Identifiers

  • HAL Id : hal-00189174 , version 1
  • PUBMED : 17589922

Cite

Juan-Enrique Tichauer, Maria-Gabriela Morales, Ludwig Amigo, Leopoldo Galdames, Andres Klein, et al.. Overexpression of the cholesterol-binding protein MLN64 induces liver damage in the mouse.. World Journal of Gastroenterology, 2007, 13 (22), pp.3071-9. ⟨hal-00189174⟩
129 View
0 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More