Expression of a putative plant viral gene in Escherichia coli. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Gene Année : 1984

Expression of a putative plant viral gene in Escherichia coli.

A. Geldreich
  • Fonction : Auteur
C. Xiong
  • Fonction : Auteur
G. Lebeurier
  • Fonction : Auteur
L. Hirth
  • Fonction : Auteur

Résumé

A recombinant plasmid, pCB300, was constructed which carries a cauliflower mosaic virus (CaMV) DNA insert corresponding to nucleotides 1825-2280, including the coding sequence (1830-2219) of open reading frame III (ORF III). This CaMV DNA insert was fused with the amino-terminal portion of the beta-galactosidase gene. Transcription of the hybrid gene is controlled by the lac promoter, which is repressed in Escherichia coli strain JM103 and can be induced by isopropylthio-beta-D-galactoside (IPTG). When the promoter is derepressed, cells harboring the chimeric plasmid produce an Mr 16 000 fusion protein. This protein is immunodetected by antibodies raised against an amino terminal synthetic peptide of 19 amino acids corresponding to a sequence predicted from the nucleotide sequence of ORF III.

Domaines

Virologie
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Dates et versions

hal-00189173 , version 1 (20-11-2007)

Identifiants

  • HAL Id : hal-00189173 , version 1
  • PUBMED : 6098536

Citer

Jean-Michel Mesnard, A. Geldreich, C. Xiong, G. Lebeurier, L. Hirth. Expression of a putative plant viral gene in Escherichia coli.. Gene, 1984, 31 (1-3), pp.39-47. ⟨hal-00189173⟩
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