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Journal Articles Genesis Year : 2005

Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse.

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Abstract

To generate temporally controlled targeted somatic mutations selectively and efficiently in skeletal muscles, we established a transgenic HSA-Cre-ER(T2) mouse line in which the expression of the tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the human skeletal muscle alpha-actin gene, contained in a P1-derived artificial chromosome. In this transgenic line Cre-ER(T2) is selectively expressed in skeletal muscles, and Cre-ER(T2)-mediated alteration of LoxP flanked (floxed) target genes is skeletal muscle-specific and strictly tamoxifen-dependent. HSA-Cre-ER(T2) mice should be of great value to analyze gene function in skeletal muscles, and to establish animal models of human skeletal muscle disorders.

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hal-00187752 , version 1 (15-11-2007)

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Michael Schuler, Faisal Ali, Elisabeth Metzger, Pierre Chambon, Daniel Metzger. Temporally controlled targeted somatic mutagenesis in skeletal muscles of the mouse.. Genesis, 2005, 41 (4), pp.165-70. ⟨10.1002/gene.20107⟩. ⟨hal-00187752⟩
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