Targeted somatic mutagenesis in the mouse epidermis. - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2005

Targeted somatic mutagenesis in the mouse epidermis.

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Abstract

The efficient introduction of somatic mutations in a given gene at a given time and in specific cell types of the skin will greatly facilitate the studies of a number of genes expressed in the skin and the production of animal models for skin diseases. We describe here strategies and techniques to create spatiotemporally controlled somatic mutations of target genes in the skin using a conditional Cre/LoxP system. They are based on cell-specific expression of the chimeric Cre recombinase Cre-ERT2, whose activity is induced by antiestrogens such as Tamoxifen (Tam), and which is obtained by fusing the Cre recombinase with a mutated ligand binding domain of the human estrogen receptor ERalpha. As an example, we present ablation of the retinoid receptor RXRalpha in epidermal basal keratinocytes of adult mice.
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Dates and versions

hal-00187700 , version 1 (15-11-2007)

Identifiers

  • HAL Id : hal-00187700 , version 1
  • PUBMED : 15502196

Cite

Daniel Metzger, Mei Li, Pierre Chambon. Targeted somatic mutagenesis in the mouse epidermis.. Methods in Molecular Biology, 2005, 289, pp.329-40. ⟨hal-00187700⟩
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