Dental Epithelial Histomorphogenesis in vitro. - Archive ouverte HAL Access content directly
Journal Articles Journal of Dental Research Year : 2005

Dental Epithelial Histomorphogenesis in vitro.

B. Hu
  • Function : Author
A. Nadiri
  • Function : Author
S. Bopp-Küchler
  • Function : Author
H. Lesot
  • Function : Author


Recent developments in tooth-tissue engineering require that we understand the regulatory processes to be preserved to achieve histomorphogenesis and cell differentiation, especially for enamel tissue engineering. Using mouse first lower molars, our objectives were: (1) to determine whether the cap-stage dental mesenchyme can control dental epithelial histogenesis, (2) to test the role of the primary enamel knot (PEK) in specifying the potentialities of the dental mesenchyme, and (3) to evaluate the importance of positional information in epithelial cells. After tissue dissociation, the dental epithelium was further dissociated into individual cells, re-associated with dental mesenchyme, and cultured. Epithelial cells showed a high plasticity: Despite a complete loss of positional information, they rapidly underwent typical dental epithelial histogenesis. This was stimulated by the mesenchyme. Experiments performed at E13 demonstrated that the initial potentialities of the mesenchyme are not specified by the PEK. Positional information of dental epithelial cells does not require the memorization of their history.
No file

Dates and versions

hal-00187627 , version 1 (15-11-2007)


  • HAL Id : hal-00187627 , version 1
  • PUBMED : 15914588


B. Hu, A. Nadiri, S. Bopp-Küchler, F. Perrin-Schmitt, H. Lesot. Dental Epithelial Histomorphogenesis in vitro.. Journal of Dental Research, 2005, 84 (6), pp.521-5. ⟨hal-00187627⟩
29 View
0 Download



Gmail Mastodon Facebook X LinkedIn More