Identification of ribosome-associated viral and cellular basic proteins during the course of infection with herpes simplex virus type 1. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proteomics Année : 2001

Identification of ribosome-associated viral and cellular basic proteins during the course of infection with herpes simplex virus type 1.

Résumé

Herpes simplex virus type 1 (HSV-1) infection induces severe alterations of the translational apparatus, including the phosphorylation of a few ribosomal proteins, and the progressive association of several nonribosomal proteins to ribosomes. Therefore, we hypothesized that ribosomes themselves could contribute to the HSV-1-induced translational control of host and viral gene expression. As a prerequisite to test this hypothesis, we undertook the identification of the nonribosomal proteins associated to the ribosomes during the course of HSV-1 infection. After separation by two-dimensional polyacrylamide gel electrophoresis of basic proteins extracted from the ribosomal fraction, the identification of unknown protein spots was carried out by N-terminal sequencing and peptide mass determination by mass spectrometry. This allowed us to identify HSV-1 VP19C and VP26 that associated to ribosomes with different kinetics. Another nonribosomal protein turned out to be the poly(A)-binding protein 1 (PAB1P). Newly synthesized PAB1P continued to associate to ribosomes all along infection.

Dates et versions

hal-00180266 , version 1 (18-10-2007)

Identifiants

Citer

A. Greco, W. Bienvenut, J. C. Sanchez, K. Kindbeiter, D. Hochstrasser, et al.. Identification of ribosome-associated viral and cellular basic proteins during the course of infection with herpes simplex virus type 1.. Proteomics, 2001, 1 (4), pp.545-9. ⟨10.1002/1615-9861(200104)1:4<545::AID-PROT545>3.0.CO;2-G⟩. ⟨hal-00180266⟩
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