TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Genesis Année : 2007

TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis.

Résumé

Xenopus is a well proven model for a wide variety of developmental studies, including cell lineage. Cell lineage in Xenopus has largely been addressed by injection of tracer molecules or by micro-dissection elimination of blastomeres. Here we describe a genetic method for cell ablation based on the use of tBid, a direct activator of the mitochondrial apoptotic pathway. In mammalian cells, cross-talk between the main apoptotic pathways (the mitochondrial and the death domain protein pathways) involve the pro-death protein BID, the active form of which, tBID, results from protease truncation and translocation to mitochondria. In transgenic Xenopus, restricting tBID expression to the lens-forming cells enables the specific ablation of the lens without affecting the development of other eye structures. Thus, overexpression of tBid can be used in vivo as a tool to eliminate a defined cell population by apoptosis in a developing organism and to evaluate the degree of autonomy or the inductive effects of a specific tissue during embryonic development.

Dates et versions

hal-00179423 , version 1 (15-10-2007)

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Citer

D. Du Pasquier, A. Chesneau, Q. Ymlahi-Ouazzani, R. Boistel, N. Pollet, et al.. TBid mediated activation of the mitochondrial death pathway leads to genetic ablation of the lens in Xenopus laevis.. Genesis, 2007, 45 (1), pp.1-10. ⟨10.1002/dvg.20252⟩. ⟨hal-00179423⟩
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