Improved methods for producing outer membrane vesicles in Gram-negative bacteria. - Archive ouverte HAL Access content directly
Journal Articles Research in Microbiology Year : 2004

Improved methods for producing outer membrane vesicles in Gram-negative bacteria.

Abstract

Outer membrane vesicle formation occurs during Gram-negative bacterial growth. However, natural production of large amounts of outer membrane vesicles has only been described in a few bacterial genera. The purified vesicles of some bacterial pathogens have shown potential applications in vaccinology and in antibiotic therapy. This study focused on the development of a gene expression system able to induce production of large amounts of outer membrane vesicles. The Tol-Pal system of Escherichia coli, required to maintain outer membrane integrity, is composed of five cell envelope proteins, TolA, TolB, TolQ, TolR and Pal. Tol proteins are parasitized by filamentous bacteriophages and by colicins. The phage infection process and colicin import require, respectively, the N-terminal domain of the minor coat g3p protein and the translocation domain of colicins, with both domains interacting with Tol proteins. In this study, we show that the periplasmic production of either Tol, g3p or colicin domains was able to specifically destabilize the E. coli or Shigella flexneri cell envelope and to induce production of high amounts of vesicles. This technique was further found to work efficiently in Salmonella enterica serovar Typhimurium.

Dates and versions

hal-00178521 , version 1 (11-10-2007)

Identifiers

Cite

Thomas Henry, Stéphanie Pommier, Laure Journet, Alain Bernadac, Jean-Pierre Gorvel, et al.. Improved methods for producing outer membrane vesicles in Gram-negative bacteria.. Research in Microbiology, 2004, 155 (6), pp.437-46. ⟨10.1016/j.resmic.2004.04.007⟩. ⟨hal-00178521⟩
76 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More