L-methionine degradation pathway in Kluyveromyces lactis: identification and functional analysis of the genes encoding L-methionine aminotransferase. - Archive ouverte HAL Access content directly
Journal Articles Applied and Environmental Microbiology Year : 2006

L-methionine degradation pathway in Kluyveromyces lactis: identification and functional analysis of the genes encoding L-methionine aminotransferase.

Abstract

Kluyveromyces lactis is one of the cheese-ripening yeasts and is believed to contribute to the formation of volatile sulfur compounds (VSCs) through degradation of L-methionine. L-methionine aminotransferase is potentially involved in the pathway that results in the production of methanethiol, a common precursor of VSCs. Even though this pathway has been studied previously, the genes involved have never been studied. In this study, on the basis of sequence homology, all the putative aminotransferase-encoding genes from K. lactis were cloned in an overproducing vector, pCXJ10, and their effects on the production of VSCs were analyzed. Two genes, KlARO8.1 and KlARO8.2, were found to be responsible for L-methionine aminotransferase activity. Transformants carrying these genes cloned in the pCXJ10 vector produced threefold-larger amounts of VSCs than the transformant containing the plasmid without any insert or other related putative aminotransferases produced.

Dates and versions

hal-00164087 , version 1 (19-07-2007)

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Dafni-Maria Kagkli, Pascal Bonnarme, Cécile Neuvéglise, Timothy M Cogan, Serge Casaregola. L-methionine degradation pathway in Kluyveromyces lactis: identification and functional analysis of the genes encoding L-methionine aminotransferase.. Applied and Environmental Microbiology, 2006, 72 (5), pp.3330-5. ⟨10.1128/AEM.72.5.3330-3335.2006⟩. ⟨hal-00164087⟩
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