Oxygen regulation of a nodule-located carbonic anhydrase in alfalfa. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Physiology Année : 2000

Oxygen regulation of a nodule-located carbonic anhydrase in alfalfa.

S. Gálvez
  • Fonction : Auteur
A. M. Hirsch
  • Fonction : Auteur
K. L. Wycoff
  • Fonction : Auteur
S. Hunt
  • Fonction : Auteur
D. B. Layzell
  • Fonction : Auteur
M. Crespi

Résumé

Control of the permeability to oxygen is critical for the function of symbiotic nitrogen fixation in legume nodules. The inner cortex (IC) seems to be a primary site for this regulation. In alfalfa (Medicago sativa) nodules, expression of the Msca1 gene encoding a carbonic anhydrase (CA) was previously found to be restricted to the IC. We have now raised antibodies against recombinant Msca1 protein and used them, together with antibodies raised against potato leaf CA, to demonstrate the presence of two forms of CA in mature nodules. Each antibody recognizes a different CA isoform in nodule tissues. Immunolocalization revealed that leaf-related CAs were localized primarily in the nitrogen-fixing zone, whereas the Msca1 protein was restricted exclusively to the IC region, in indeterminate and determinate nodules. In alfalfa nodules grown at various O(2) concentrations, an inverse correlation was observed between the external oxygen pressure and Msca1 protein content in the IC, the site of the putative diffusion barrier. Thus Msca1 is a molecular target of physiological processes occurring in the IC cells involved in gas exchange in the nodule.
Fichier non déposé

Dates et versions

hal-00162981 , version 1 (16-07-2007)

Identifiants

  • HAL Id : hal-00162981 , version 1
  • PUBMED : 11080283

Citer

S. Gálvez, A. M. Hirsch, K. L. Wycoff, S. Hunt, D. B. Layzell, et al.. Oxygen regulation of a nodule-located carbonic anhydrase in alfalfa.. Plant Physiology, 2000, 124 (3), pp.1059-68. ⟨hal-00162981⟩
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More