Evaluation of GEMSP effects on the central nervous system of the Lewis 1A rats in a chronic EAE model. - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2006

Evaluation of GEMSP effects on the central nervous system of the Lewis 1A rats in a chronic EAE model.

A. Mangas
  • Fonction : Auteur
R. Covenas
  • Fonction : Auteur
D. Bodet
  • Fonction : Auteur
M. de Leon
  • Fonction : Auteur
M.P. Dabadie
  • Fonction : Auteur

Résumé

In order to evaluate the effects of a new potential therapy in Multiple Sclerosis, we used a chronic Experimental Autoimmune Encephalomyelitis (EAE) model induced with a mixture of Myelin Oligodendrocyte Protein and Mycobacterium tuberculosis in 10 weeks old Lewis 1A female rats. A potential drug (GEMSP), was constituted of fatty acids linked to poly-lysine (PL), antioxidants linked to PL, scavengers linked to PL and amino acids linked to PL. Experiments were conducted on three groups: 1) rats with an EAE treated with GEMSP (40mg/kg/day); 2) rats with an EAE treated with NaCl (positive control); 3) rats without EAE (negative control) and treated with NaCl. The three groups were treated under the same conditions; it means that all the groups received two subcutaneous injections per day of 250µl of its respective solutions (six days a week, starting 9 days after induction of EAE). The usual clinical score (0-4) was evaluated six days a week from the first day until the day of perfusion (both included). Crises and the clinical scores were completely abolished by this potential therapy drug (GEMSP). Moreover, indirect immunocytochemical techniques were applied to evaluate leucocyte infiltration and demyelination in the rat CNS of the three groups mentioned above. Thus, the panleucocytic marker CD 45 shows a very large infiltration in the animals perfused belonging to the positive control group in the first crises; but at the end of the experiment (two months later) animals remained with clinical signs but in general no large infiltrations were found, since only some animals of the positive control group presented large infiltrations in some spinal cord levels and in the cerebellum. The anti-Myelin Basic Protein antibodies showed a lower immunoreactivity in the positive control group than in the other two groups (GEMSP and negative control). Moreover, data reported from histological techniques (Luxol Fast Blue) showed a disorganization in some levels of the spinal cord white matter only in the positive control group. Our studies clearly show that: 1) GEMSP was very active on leucocyte infiltration; 2) GEMSP may be effective on blood brain barrier rendering this less permeable to leucocyte infiltration, decreasing the leucocyte infiltration per se, and could play an immunomodulator role; 3) GEMSP abolish EAE crisis; 4) GEMSP seems to have a myelin protection
Fichier non déposé

Dates et versions

hal-00162048 , version 1 (12-07-2007)

Identifiants

  • HAL Id : hal-00162048 , version 1

Citer

A. Mangas, R. Covenas, D. Bodet, M. de Leon, M. Geffard, et al.. Evaluation of GEMSP effects on the central nervous system of the Lewis 1A rats in a chronic EAE model.. 16th ENS Meeting, May 2006, Lausanne, Switzerland. ⟨hal-00162048⟩
116 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More