(15)N-Metabolic labeling for comparative plasma membrane proteomics in Arabidopsis cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proteomics Année : 2007

(15)N-Metabolic labeling for comparative plasma membrane proteomics in Arabidopsis cells.

Résumé

An important goal for proteomic studies is the global comparison of proteomes from different genotypes, tissues, or physiological conditions. This has so far been mostly achieved by densitometric comparison of spot intensities after protein separation by 2-DE. However, the physicochemical properties of membrane proteins preclude the use of 2-DE. Here, we describe the use of in vivo labeling by the stable isotope (15)N as an alternative approach for comparative membrane proteomic studies in plant cells. We confirm that (15)N-metabolic labeling of proteins is possible and efficient in Arabidopsis suspension cells. Quantification of (14)N versus (15)N MS signals reflects the relative abundance of (14)N and (15)N proteins in the sample analyzed. We describe the use of (15)N-metabolic labeling to perform a partial comparative analysis of Arabidopsis cells following cadmium exposure. By focusing our attention on plasma membrane proteins, we were able to confidently identify proteins showing up to 5-fold regulation compared to unexposed cells. This study provides a proof of principle that (15)N-metabolic labeling is a useful technique for comparative membrane proteome studies.

Dates et versions

hal-00139621 , version 1 (02-04-2007)

Identifiants

Citer

Viviane Lanquar, Lauriane Kuhn, Françoise Lelièvre, Mehdi Khafif, Christelle Espagne, et al.. (15)N-Metabolic labeling for comparative plasma membrane proteomics in Arabidopsis cells.. Proteomics, 2007, 7 (5), pp.750-754. ⟨10.1002/pmic.200600791⟩. ⟨hal-00139621⟩
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