Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Plant Journal Année : 2003

Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula.

Résumé

The tobacco element, Tnt1, is one of the few active retrotransposons in plants. Its transposition is activated during protoplast culture in tobacco and tissue culture in the heterologous host Arabidopsis thaliana. Here, we report its transposition in the R108 line of Medicago truncatula during the early steps of the in vitro transformation-regeneration process. Two hundred and twenty-five primary transformants containing Tnt1 were obtained. Among them, 11.2% contained only transposed copies of the element, indicating that Tnt1 transposed very early and efficiently during the in vitro transformation process, possibly even before the T-DNA integration. The average number of insertions per transgenic line was estimated to be about 15. These insertions were stable in the progeny and could be separated by segregation. Inspection of the sequences flanking the insertion sites revealed that Tnt1 had no insertion site specificity and often inserted in genes (one out of three insertions). Thus, our work demonstrates the functioning of an efficient transposable element in leguminous plants. These results indicate that Tnt1 can be used as a powerful tool for insertion mutagenesis in M. truncatula.

Dates et versions

hal-00134885 , version 1 (05-03-2007)

Identifiants

Citer

Isabelle d'Erfurth, Viviane Cosson, Alexis Eschstruth, Helene Lucas, Adam Kondorosi, et al.. Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula.. The Plant Journal, 2003, 34 (1), pp.95-106. ⟨10.1046/j.1365-313X.2003.01701.x⟩. ⟨hal-00134885⟩
158 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More