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Article Dans Une Revue Biology of the Cell Année : 2006

Multiple-axis tomography: applications to basal bodies from Paramecium tetraurelia.

Résumé

BACKGROUND INFORMATION: Transmission electron tomography is becoming a powerful tool for studying subcellular components of cells. Classical approaches for electron tomography consist of recording images along a single-tilt axis. This approach is being improved by dual-axis reconstructions and/or high-tilt devices (tilt angle>+/-60 degrees) on microscopes to compensate part of the information loss due to the 'missing wedge' phenomena. RESULTS: In the present work we have evaluated the extension of the dual-axis technique to a multiple-axis approach, and we demonstrate a freely available plug-in for the Java-based freeware image-analysis software ImageJ. Our results from phantom and experimental data sets from Paramecium tetraurelia epon-embedded sections have shown that multiple-axis tomography achieves results equivalent to those obtained by dual-axis approach without the requirement for high-tilt devices. CONCLUSIONS: This new approach allows performance of high-resolution tomography, avoiding the need for high-tilt devices, and therefore will increase the access of electron tomography to a larger community.

Dates et versions

hal-00133088 , version 1 (23-02-2007)

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Cédric Messaoudi, Nicole Garreau de Loubresse, Thomas Boudier, Pascale Dupuis-Williams, Sergio Marco. Multiple-axis tomography: applications to basal bodies from Paramecium tetraurelia.. Biology of the Cell, 2006, 98 (7), pp.415-25. ⟨10.1042/BC20050097⟩. ⟨hal-00133088⟩
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