Generation of specific Ca(2+) signals from Ca(2+) stores and endocytosis by differential coupling to messengers. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Current Biology - CB Année : 2006

Generation of specific Ca(2+) signals from Ca(2+) stores and endocytosis by differential coupling to messengers.

Résumé

It remains unclear how different intracellular stores could interact and be recruited by Ca(2+)-releasing messengers to generate agonist-specific Ca(2+) signatures. In addition, refilling of acidic stores such as lysosomes and secretory granules occurs through endocytosis, but this has never been investigated with regard to specific Ca(2+) signatures. In pancreatic acinar cells, acetylcholine (ACh), cholecystokinin (CCK), and the messengers cyclic ADP-ribose (cADPR), nicotinic acid adenine dinucleotide phosphate (NAADP), and inositol 1,4,5-trisphosphate (IP(3)) evoke repetitive local Ca(2+) spikes in the apical pole. Our work reveals that local Ca(2+) spikes evoked by different agonists all require interaction of acid Ca(2+) stores and the endoplasmic reticulum (ER), but in different proportions. CCK and ACh recruit Ca(2+) from lysosomes and from zymogen granules through different mechanisms; CCK uses NAADP and cADPR, respectively, and ACh uses Ca(2+) and IP(3), respectively. Here, we provide pharmacological evidence demonstrating that endocytosis is crucial for the generation of repetitive local Ca(2+) spikes evoked by the agonists and by NAADP and IP(3). We find that cADPR-evoked repetitive local Ca(2+) spikes are particularly dependent on the ER. We propose that multiple Ca(2+)-releasing messengers determine specific agonist-elicited Ca(2+) signatures by controlling the balance among different acidic Ca(2+) stores, endocytosis, and the ER.

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Domaines

Neurobiologie

Dates et versions

hal-00117276 , version 1 (30-11-2006)

Identifiants

Citer

Alexis Menteyne, Anton Burdakov, Gilles Charpentier, Ole H Petersen, José-Manuel Cancela. Generation of specific Ca(2+) signals from Ca(2+) stores and endocytosis by differential coupling to messengers.. Current Biology - CB, 2006, 16 (19), pp.1931-1937. ⟨10.1016/j.cub.2006.07.070⟩. ⟨hal-00117276⟩
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