Characterization of the Drosophila myeloid leukemia factor. - Archive ouverte HAL
Article Dans Une Revue Genes to Cells Année : 2006

Characterization of the Drosophila myeloid leukemia factor.

Résumé

In human, the myeloid leukemia factor 1 (hMLF1) has been shown to be involved in acute leukemia, and mlf related genes are present in many animals. Despite their extensive representation and their good conservation, very little is understood about their function. In Drosophila, dMLF physically interacts with both the transcription regulatory factor DREF and an antagonist of the Hedgehog pathway, Suppressor of Fused, whose over-expression in the fly suppresses the toxicity induced by polyglutamine. No connection between these data has, however, been established. Here, we show that dmlf is widely and dynamically expressed during fly development. We isolated and analyzed the first dmlf mutants: embryos lacking maternal dmlf product have a low viability with no specific defect, and dmlf(-)- adults display weak phenotypes. We monitored dMLF subcellular localization in the fly and cultured cells. We were able to show that, although generally nuclear, dMLF can also be cytoplasmic, depending on the developmental context. Furthermore, two differently spliced variants of dMLF display differential subcellular localization, allowing the identification of regions of dMLF potentially important for its localization. Finally, we demonstrate that dMLF can act developmentally and postdevelopmentally to suppress neurodegeneration and premature aging in a cerebellar ataxia model.

Dates et versions

hal-00116627 , version 1 (27-11-2006)

Identifiants

Citer

Séverine Martin-Lannerée, Christelle Lasbleiz, Matthieu Sanial, Sylvaine Fouix, Florence Besse, et al.. Characterization of the Drosophila myeloid leukemia factor.. Genes to Cells, 2006, 11 (12), pp.1317-35. ⟨10.1111/j.1365-2443.2006.01023.x⟩. ⟨hal-00116627⟩
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