Genetic characterization of thymidine kinase from acyclovir-resistant and -susceptible herpes simplex virus type 1 isolated from bone marrow transplant recipients. - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2006

Genetic characterization of thymidine kinase from acyclovir-resistant and -susceptible herpes simplex virus type 1 isolated from bone marrow transplant recipients.

F. Morfin
G. Souillet
  • Fonction : Auteur
K. Bilger
  • Fonction : Auteur
M. Aymard
  • Fonction : Auteur

Résumé

Emergence of acyclovir (Acy)-resistant herpes simplex virus (HSV) is a major concern in bone marrow transplant recipients. Phenotypic and genetic characterization of thymidine kinase (TK) was done for 7 Acy-susceptible and 11 Acy-resistant HSV-1 isolated from 11 patients. In total, 19 amino acid substitutions were detected that were not related to Acy resistance but to TK gene polymorphism, including 5 mutations that have not been previously reported. The Acy-resistant strain from 1 patient presented no TK gene mutation related to resistance. Five patients (45%) had isolates that harbored point mutations leading to amino acid substitutions that could be associated with Acy resistance. Of the 5 substitutions detected, 3 have not been previously reported (codons 51, 83, and 175). A nucleotide insertion or deletion was detected in resistant isolates from 5 patients (45%); these mutations are located in homopolymer repeats at codon 92 (1 subject) and at codon 146 (4 subjects).
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Dates et versions

hal-00115856 , version 1 (23-11-2006)

Identifiants

  • HAL Id : hal-00115856 , version 1
  • PUBMED : 10882609

Citer

F. Morfin, G. Souillet, K. Bilger, T. Ooka, M. Aymard, et al.. Genetic characterization of thymidine kinase from acyclovir-resistant and -susceptible herpes simplex virus type 1 isolated from bone marrow transplant recipients.. 2006. ⟨hal-00115856⟩
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