Expression of BARF1 gene encoded by Epstein-Barr virus in nasopharyngeal carcinoma biopsies. - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2006

Expression of BARF1 gene encoded by Epstein-Barr virus in nasopharyngeal carcinoma biopsies.

Résumé

We reported previously that the EBV BARF1 open reading frame encodes a Mr 31,000-33,000 protein (p31) with potential transforming and oncogenic properties. This gene was found capable of transforming both: (a) the rodent fibroblast lines Balbc/3T3 and NIH3T3 into cells producing aggressive tumors in newborn rats; and (b) the human EBV-negative B-cell line Louckes into cells leading to small tumors, which disappeared 3 weeks after injection. Our recent study showed that BARF1 ORF expression may confer the property of immortalization to primary kidney epithelial cells (M. X. Wei et al., Oncogene, 14: 3073-3081, 1997). Because this suggested that BARF1 could be involved in epithelial malignancy, we investigated its transcriptional and translational expressions in Algerian nasopharyngeal carcinoma (NPC) biopsies by reverse transcription-PCR and immunoblotting using rabbit polyclonal antisera prepared against two synthetic peptides corresponding to distinct, predicted epitopes of the BARF1 protein (NGGVMKEKD, amino acids 172-180, and GKNDKEE, amino acids 203-209). The BARF1 ORF was found to be transcribed and translated in >85% of our NPC biopsies, with high p31 protein level detected in several NPC patient biopsies as well as in NPC-derived xenografts. Our observation of BARF1 expression in a large proportion of NPC epithelial cells suggests that this EBV gene might play an important role in the malignant transformation of human epithelial cells in vivo.
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Dates et versions

hal-00115841 , version 1 (23-11-2006)

Identifiants

  • HAL Id : hal-00115841 , version 1
  • PUBMED : 11034107

Citer

G. Decaussin, F. Sbih-Lammali, M. de Turenne-Tessier, A. Bouguermouh, T. Ooka. Expression of BARF1 gene encoded by Epstein-Barr virus in nasopharyngeal carcinoma biopsies.. 2006. ⟨hal-00115841⟩
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