Multiple levels of interactions within the tetraspanin web.
Résumé
The tetraspanin web refers to a network of molecular interactions involving tetraspanins and other molecules. Inside the tetraspanin web, small primary complexes containing only one tetraspanin and one specific partner molecule such as CD151/alpha3beta1 integrin and CD9/CD9P-1 (FPRP) can be observed under particular conditions. Here we demonstrate that when cells are lysed with Brij97, the tetraspanins CD151 and CD9 allow and/or stabilize the interaction of their partner molecules with other tetraspanins and that their two partners associate under conditions maintaining tetraspanin/tetraspanin interactions. The tetraspanins were also found to partition into a detergent-resistant membrane environment to which the integrin alpha3beta1 was relocalized upon expression of CD151.
Mots clés
Animals
Antigens
CD/*metabolism
Differentiation
T-Lymphocyte/metabolism
Carrier Proteins/metabolism
Cell Line
Cricetinae
Detergents/chemistry
Humans
Integrin alpha3beta1/analysis/metabolism
Macromolecular Substances
Membrane Glycoproteins/metabolism
Membrane Microdomains/chemistry
Membrane Proteins/metabolism
Models
Biological
Neoplasm Proteins/metabolism
Research Support
Non-U.S. Gov't