Internalization of a GFP-tetanus toxin C-terminal fragment fusion protein at mature mouse neuromuscular junctions. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Neuroscience Année : 2005

Internalization of a GFP-tetanus toxin C-terminal fragment fusion protein at mature mouse neuromuscular junctions.

Résumé

The distribution, dynamics, internalization, and retrograde axonal traffic of a fusion protein composed of green fluorescent protein (GFP)and the atoxic C-terminal fragment of tetanus toxin (TTC) were studied after its in vivo injection. Confocal microscopy and immuno-gold electron microscopy revealed that the fusion protein (GFP-TTC) rapidly clustered in motor nerve terminals of the neuromuscular junction. Clathrin-coated pits, and axolemma infoldings located between active zones appeared to be involved in the internalization of the fusion protein. Biochemical analysis of detergent-extracted neuromuscular preparations showed that the GFP-TTC fusion protein was associated with lipid microdomains. We suggest that GFP-TTC clustering in these lipid microdomains favors the recruitment of other proteins involved in its endocytosis and internalization in motor nerve terminals. During its retrograde trafficking, GFP-TTC accumulated indifferent axonal compartments than those used by cholera toxin B-subunit suggesting that these two proteins are transported by different pathways and cargos.

Domaines

Neurobiologie

Dates et versions

hal-00109564 , version 1 (24-10-2006)

Identifiants

Citer

Sylvie Roux, Cesare Colasante, Cécile Saint Cloment, Julien Barbier, Thomas Curie, et al.. Internalization of a GFP-tetanus toxin C-terminal fragment fusion protein at mature mouse neuromuscular junctions.. Molecular and Cellular Neuroscience, 2005, 30 (4), pp.572-582. ⟨10.1016/j.mcn.2005.05.008⟩. ⟨hal-00109564⟩

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