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Article Dans Une Revue FEBS Letters Année : 2004

beta-Dystroglycan can be revealed in microsomes from mdx mouse muscle by detergent treatment

Résumé

beta-Dystroglycan is the central member of a transmembrane protein complex of the skeletal muscle plasma membrane. Since it was not detected in dystrophin-deficient skeletal muscles, a disruption of the complex was thought to be involved in the dystrophic process. We report here that beta-dystroglycan is actually present at normal levels in mdx mouse muscle plasma membrane: treatment with cholate detergent is able to reveal its presence by SDS-PAGE and immunoblotting. This result shows that, in dystrophin-deficient muscles, beta-dystroglycan is indeed targeted to the plasma membrane but remains inaccessible to classical solubilizing treatments and to antibodies used for immunolocalization

Dates et versions

hal-00108426 , version 1 (20-10-2006)

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Citer

Nicolas Cluchague, Céline Moreau, Chantal Rocher, Sandrine Pottier, Geneviève Leray, et al.. beta-Dystroglycan can be revealed in microsomes from mdx mouse muscle by detergent treatment. FEBS Letters, 2004, 572 (1-3), pp.216-220. ⟨10.1016/j.febslet.2004.07.032⟩. ⟨hal-00108426⟩
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