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Article Dans Une Revue Journal of the American Chemical Society Année : 2003

Resolution Enhancement in Multidimensional Solid-State NMR Spectroscopy of Proteins using Spin-State Selection

Résumé

A new experimental approach is introduced which leads to significant resolution enhancement in multidimensional 13C-13C correlation experiments of microcrystalline systems. Spin-state-selective techniques, adapted for solid-state NMR, are used for removing the J-coupling contribution to the 13C lineshapes. Combination of the spin-state-selective elements and standard ZQ or DQ solid-state NMR mixing sequences allows to perform a spin-state-selective polarization transfer. In addition to the resolution improvement, the new technique enables to distinguish "direct" cross peaks involving covalently bound nuclei from "relayed" cross peaks.
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Dates et versions

hal-00078170 , version 1 (05-06-2006)

Identifiants

Citer

Luminita Duma, Sabine Hediger, Bernhard Brutscher, Anja Böckmann, Lyndon Emsley. Resolution Enhancement in Multidimensional Solid-State NMR Spectroscopy of Proteins using Spin-State Selection. Journal of the American Chemical Society, 2003, 125, pp.11816. ⟨10.1021/ja036893n⟩. ⟨hal-00078170⟩
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