Light chain inclusion permits terminal B cell differentiation and does not necessarily result in autoreactivity. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2006

Light chain inclusion permits terminal B cell differentiation and does not necessarily result in autoreactivity.

Résumé

Mice in which the Jkappa cluster was replaced with a VkappaJkappa rearranged gene were studied. More than 90% of B cells from homozygous mutant mice expressed the transgenic kappa chain but showed a slightly reduced level of kappa transcripts compared with WT B lymphocytes. Light chain inclusion was apparent in 10% of B cells from these mice and raised 25% in hemizygous mice with a still lower expression of the knockin kappa chain. Beyond the rules of clonal selection, peripheral B cells developed in such animals, with included cells being activated and differentiating into class-switched or antibody-secreting cells. The high amount of included mature B cells was associated with an increase of hybrid kappa/lambda immunoglobulins but not with the increased prevalence of autoantibodies. Altogether, these data suggest that light chain exclusion prevalent in normal B cells mostly results from ordered rearrangements and stochastic mechanisms but is neither tightly ensured by a stringent cell selection process nor absolutely required for normal B cell function.

Domaines

Immunologie

Dates et versions

hal-00073857 , version 1 (24-05-2006)

Identifiants

Citer

Christophe Sirac, Claire Carrion, Sophie Duchez, Isabelle Comte, Michel Cogné. Light chain inclusion permits terminal B cell differentiation and does not necessarily result in autoreactivity.. Proceedings of the National Academy of Sciences of the United States of America, 2006, 103 (20), pp.7747-52. ⟨10.1073/pnas.0509121103⟩. ⟨hal-00073857⟩
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