The beta-globin HS4 insulator confers copy-number dependent expression of IgH regulatory elements in stable B cell transfectants. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Immunology Letters Année : 2003

The beta-globin HS4 insulator confers copy-number dependent expression of IgH regulatory elements in stable B cell transfectants.

Résumé

Locus control regions (LCR) were first defined by their theoretical ability to enhance the expression of linked genes in a tissue-specific, position-independent and copy-number dependent manner. In fact, few of the so-called LCR identified completely fulfil this definition. For example, the regulatory elements located in 5' (Emu) and 3' (HS3a; HS1,2; HS3b; HS4) of the IgH locus display some properties of a LCR but lack a copy-number dependence and sometimes display position effects in transgenes. In order to study whether addition of insulators would allow to overcome such problems in transgenes, we studied constructs harboring a V(H) promoter-green fluorescent protein reporter gene linked to the 3' and/or 5' IgH elements, surrounded or not with the chicken beta-globin 5'HS4 insulator. When flanked with insulators it appeared that either 3' IgH and 5' IgH regulatory elements now behave as true LCR elements and noticeably display copy-number dependence in transfected pre-B or B cell lines.

Domaines

Immunologie

Dates et versions

hal-00023379 , version 1 (25-04-2006)

Identifiants

Citer

Laurence Guglielmi, Véronique Truffinet, Michel Cogné, Yves Denizot. The beta-globin HS4 insulator confers copy-number dependent expression of IgH regulatory elements in stable B cell transfectants.. Immunology Letters, 2003, 89 (2-3), pp.119-23. ⟨10.1016/S0165-2478(03)00126-3⟩. ⟨hal-00023379⟩
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