Induction of somatic hypermutation by antigen-specific B cell receptors in the human BL2 cell line. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue European Journal of Immunology Année : 2004

Induction of somatic hypermutation by antigen-specific B cell receptors in the human BL2 cell line.

Résumé

The role of the B cell antigen receptor in the induction of somatic hypermutation is presently unclear. We established stable transfectants of the human BL2 cell line expressing hen-egg lysozyme-specific IgM or IgA and compared their ability to induce somatic hypermutation of the endogenous rearranged heavy-chain gene. We found that IgM and IgA were both able to induce somatic hypermutation in an antigen dose-independent manner. The mutations displayed most of the characteristics of somatic hypermutation in vivo. Notably, some replacements introduced stop codons in the coding region. Our data suggest that class-switched memory B cells may undergo somatic hypermutation. They also suggest that the transmembrane/cytoplasmic domains of the class-switched isotypes modulate the signaling and down-modulation activities of the BCR in an antigen dose-dependent manner.

Domaines

Immunologie
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Dates et versions

hal-00023350 , version 1 (25-04-2006)

Identifiants

Citer

Florence Glaudet, Vincent Denis, Michel Cogné, Ahmed Amine Khamlichi. Induction of somatic hypermutation by antigen-specific B cell receptors in the human BL2 cell line.. European Journal of Immunology, 2004, 34 (6), pp.1637-45. ⟨10.1002/eji.200324741⟩. ⟨hal-00023350⟩
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