Functional characterization of 2'-O-methylation and pseudouridylation guide RNAs - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Methods in Molecular Biology Année : 2004

Functional characterization of 2'-O-methylation and pseudouridylation guide RNAs

Tamás Kiss
  • Fonction : Auteur
  • PersonId : 906442
Beáta E Jády

Résumé

Pseudouridines and 2′-O-methylated nucleotides are ubiquitous constituents of stable cellular RNAs. In eukaryotes, posttranscriptional synthesis of most pseudouridines and 2′-O-methylated nucleotides is directed by sequence-specific guide RNAs (gRNAs). In recent years, an enormous number of novel putative modification gRNAs have been identified in a broad variety of organisms by using bioinformatics and large-scale cDNA-sequencing approaches. Understanding of the function of the novel modification gRNAs as well as the functional importance of modified nucleotides requires techniques that support the site-specific detection of 2′-O-methylated nucleotides and pseudouridines. Here, we describe rapid, reverse transcription-based methods to map 2′-O-methylated nucleotides and pseudouridines in any cellular RNA.
Fichier non déposé

Dates et versions

hal-00022397 , version 1 (07-04-2006)

Identifiants

Citer

Tamás Kiss, Beáta E Jády. Functional characterization of 2'-O-methylation and pseudouridylation guide RNAs. Methods in Molecular Biology, 2004, 265, pp.393-408. ⟨10.1385/1-59259-775-0:393⟩. ⟨hal-00022397⟩
22 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More