Homer-dependent cell surface expression of metabotropic glutamate receptor type 5 in neurons - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Neuroscience Année : 2002

Homer-dependent cell surface expression of metabotropic glutamate receptor type 5 in neurons

Fabrice Ango
David Robbe
J.C. Tu
  • Fonction : Auteur
B. Xiao
  • Fonction : Auteur
P.F. Worley
  • Fonction : Auteur
Jean-Philippe Pin
Joël Bockaert
Laurent Fagni
  • Fonction : Auteur
  • PersonId : 853454

Résumé

The metabotropic glutamate (mGlu) receptors are a family of receptors involved in the tuning of fast excitatory synaptic transmission in the brain. Experiments performed in heterologous expression systems suggest that cell surface expression of group I mGlu receptors is controlled by their auxiliary protein, Homer. However, whether or not this also applies to neurons remains controversial. Here we show that in cultured cerebellar granule cells, the group I mGlu receptor subtype, mGlu5, transfected alone is functionally expressed at the surface of these neurons. Transfected Homer1b caused intracellular retention and clustering of this receptor at synaptic sites. Recombinant Homer1a alone did not affect cell surface expression of the receptor, but in neurons transfected with Homer1b, excitation-induced expression of native Homer1a reversed the intracellular retention of mGlu5 receptors, resulting in the receptor trafficking to synaptic membranes. Transfected Homer1a also increased the latency and amplitude of the mGlu5 receptor Ca2+ response. These results indicate that Homer1 proteins regulate synaptic cycling and Ca2+ signaling of mGlu5 receptors, in response to neuronal activity.

Dates et versions

hal-00016884 , version 1 (13-01-2006)

Identifiants

Citer

Fabrice Ango, David Robbe, J.C. Tu, B. Xiao, P.F. Worley, et al.. Homer-dependent cell surface expression of metabotropic glutamate receptor type 5 in neurons. Molecular and Cellular Neuroscience, 2002, 20, pp.323-329. ⟨10.1006/mcne.2002.1100⟩. ⟨hal-00016884⟩
42 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More