The Ubiquitin Ligase Rsp5p is Required for the Modification and Sorting of Biosynthetic Membrane Proteins into Multivesicular Bodies in S. cerevisiae. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Traffic (Copenhagen, Denmark) Année : 2004

The Ubiquitin Ligase Rsp5p is Required for the Modification and Sorting of Biosynthetic Membrane Proteins into Multivesicular Bodies in S. cerevisiae.

Résumé

Precursor forms of vacuolar proteins with transmembrane domains, such as the carboxypeptidase S Cps1p and the polyphosphatase Phm5p, are selectively sorted in endosomal compartments to vesicles that invaginate, budding into the lumen of the late endosomes, resulting in the formation of multivesicular bodies (MVBs). These proteins are then delivered to the vacuolar lumen following fusion of the MVBs with the vacuole. The sorting of Cps1p and Phm5p to these structures is mediated by ubiquitylation, and in a doa4 mutant, which has reduced level of free ubiquitin, these proteins are missorted to the vacuolar membrane. A RING-finger ubiquitin ligase Tul1p has been shown to participate to the ubiquitylation of Cps1p and Phm5p. We show here that the HECT-ubiquitin ligase Rsp5p is also required for the ubiquitylation of these proteins, and therefore for their sorting to MVBs. Rsp5p is an essential ubiquitin ligase containing an N-terminal C2 domain followed by three WW domains, and a C-terminal catalytic HECT domain. In cells with low levels of Rsp5p (npi1 mutant cells), vacuolar hydrolases do not reach the vacuolar lumen and are instead missorted to the vacuolar membrane. The C2 domain and the second WW domain of Rsp5p are important determinants for sorting to MVBs. Removal of the Bul proteins, two components of the Rsp5p ubiquitin ligase complex, also impairs the sorting of these proteins, but to a lesser extent. Ubiquitylation of Cps1p was strongly reduced in the npi1 mutant strain and ubiquitylation was completely abolished in the npi1 tul1 double mutant. These data demonstrate that Rsp5p plays a novel and key role in intracellular trafficking, and extend the currently very short list of substrates ubiquitylated in vivo by several different ubiquitin ligases acting cooperatively.
Fichier principal
Vignette du fichier
Morvan_et_al_Traffic_2004.pdf (472.44 Ko) Télécharger le fichier
Loading...

Dates et versions

hal-00016367 , version 1 (30-12-2005)

Identifiants

Citer

Joelle Morvan, Marine Froissard, Rosine Haguenauer-Tsapis, Danièle Urban-Grimal. The Ubiquitin Ligase Rsp5p is Required for the Modification and Sorting of Biosynthetic Membrane Proteins into Multivesicular Bodies in S. cerevisiae.. Traffic (Copenhagen, Denmark), 2004, 5, pp.383-392. ⟨10.1111/j.1600-0854.2004.00183.x⟩. ⟨hal-00016367⟩
149 Consultations
65 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More