Laser Doppler holographic microscopy in transmission: application to fish embryo imaging - Laboratoire Charles Coulomb (L2C) Access content directly
Journal Articles Optics Express Year : 2014

Laser Doppler holographic microscopy in transmission: application to fish embryo imaging

Abstract

We have extended Laser Doppler holographic microscopy to transmission geometry. The technique is validated with living fish embryos imaged by a modified upright bio-microcope. By varying the frequency of the holographic reference beam, and the combination of frames used to calculate the hologram, multimodal imaging has been performed. Doppler images of the blood vessels for different Doppler shifts, images where the flow direction is coded in RGB colors or movies showing blood cells individual motion have been obtained as well. The ability to select the Fourier space zone that is used to calculate the signal, makes the method quantitative.
Fichier principal
Vignette du fichier
MultimodalZebrafish_MG_23.pdf (905.89 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-00970195 , version 1 (30-04-2014)

Identifiers

Cite

Nicolas Verrier, Daniel Alexandre, Michel Gross. Laser Doppler holographic microscopy in transmission: application to fish embryo imaging. Optics Express, 2014, 22, pp.9368. ⟨10.1364/OE.22.009368⟩. ⟨hal-00970195⟩
82 View
239 Download

Altmetric

Share

Gmail Facebook X LinkedIn More