Collagen/polyester-polyurethane porous scaffolds for use in meniscal repair - Institut Charles Gerhardt - Institut de Chimie Moléculaire et des Matériaux de Montpellier Accéder directement au contenu
Article Dans Une Revue Biomaterials Science Année : 2024

Collagen/polyester-polyurethane porous scaffolds for use in meniscal repair

Résumé

Focusing on the regeneration of damaged knee meniscus, we propose a hybrid scaffold made of poly(ester-urethane) (PEU) and collagen that combines suitable mechanical properties with enhanced biological integration. To ensure biocompatibility and degradability, the degradable PEU was prepared from a poly(ε-caprolactone), L-lysine diisocyanate prepolymer (PCL di-NCO) and poly(lactic-co-glycolic acid) diol (PLGA). The resulting PEU (M$_n$ = 52 000 g mol$^{−1}$) was used to prepare porous scaffolds using the solvent casting (SC)/particle leaching (PL) method at an optimized salt/PEU weight ratio of 5 : 1. The morphology, pore size and porosity of the scaffolds were evaluated by SEM showing interconnected pores with a uniform size of around 170 μm. Mechanical properties were found to be close to those of the human meniscus (E$_y$ ∼ 0.6 MPa at 37°C). To enhance the biological properties, incorporation of collagen type 1 (Col) was then performed via soaking, injection or forced infiltration. The latter yielded the best results as shown by SEM-EDX and X-ray tomography analyses that confirmed the morphology and highlighted the efficient pore Col-coating with an average of 0.3 wt% Col in the scaffolds. Finally, in vitro L929 cell assays confirmed higher cell proliferation and an improved cellular affinity towards the proposed scaffolds compared to culture plates and a gold standard commercial meniscal implant
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Dates et versions

hal-04591584 , version 1 (29-05-2024)

Identifiants

Citer

Gaëlle Savin, Sylvain Caillol, Audrey Bethry, Eric Rondet, Michel Assor, et al.. Collagen/polyester-polyurethane porous scaffolds for use in meniscal repair. Biomaterials Science, 2024, 12 (11), pp.2960-2977. ⟨10.1039/d4bm00234b⟩. ⟨hal-04591584⟩
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