Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging - Ecole Nationale du Génie de l'Eau et de l'Environnement de Strasbourg Accéder directement au contenu
Article Dans Une Revue Metabolomics Année : 2019

Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging

Résumé

Introduction Micropollutants are increasingly monitored as their presence in the environment is rising due to human activities, and they are potential threats to living organisms. Objectives This study aimed at understanding the role of plants in xenobiotics removal from polluted environments by following xenobiotics metabolism in leaf tissues. Methods Different classes of micropollutants were investigated using liquid chromatography (LC) coupled to quadrupole-time of flight (Q-TOF) high resolution mass spectrometry (HRMS). The tissue localization of xenobiotics in the leaves of a spontaneous (not planted by humans) Salix alba growing near the water flux was further investigated using matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). Results The LC-Q-TOF analysis revealed the distribution of micropollutants in three different compartments of a tertiary treatment wetland. When further investing the metabolic profile of S. alba leaves using MSI, different distribution patterns were observed in specific leaf tissues. Xenobiotic metabolites were predicted and could also be tentatively identified in S. alba leaves, shedding new light on the metabolic processes at play in leaves to manage xenobiotics uptake from a polluted environment. Conclusion Using complementary metabolomics approaches, this study performed a large-scale exploration of micropollutants spreading in the environment at the exit of a tertiary treatment wetland. The use of MSI coupled with the prediction of xenobiotic metabolites yielded novel insights into plant metabolism during chronical exposure to low doses of a mixture of micropollutants.
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Dates et versions

hal-02352654 , version 1 (06-11-2019)

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Claire Villette, Loïc Maurer, Adrien Wanko, Dimitri Heintz. Xenobiotics metabolization in Salix alba leaves uncovered by mass spectrometry imaging. Metabolomics, 2019, 15, pp.122. ⟨10.1007/s11306-019-1572-8⟩. ⟨hal-02352654⟩
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