Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus. - Archive ouverte HAL Access content directly
Journal Articles Medical Mycology Year : 2002

Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus.

Isabelle Mouyna
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Jacqueline Sarfati
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Thierry Fontaine
Jean-Paul Latgé
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Abstract

A 74 kDa beta(1-3)endoglucanase of Aspergillus fumigatus was recently isolated from a cell wall autolysate and biochemically characterized. In this study, we report the cloning and the disruption of the ENGL1 gene encoding this beta(1-3)endoglucanase. ENGL1 contains an open reading frame of 2181 bp encoding a polypeptide of 727 amino acids. Sequence analysis showed that ENGL1 is the first characterized member of a new family of beta(1-3)glucanases. Disruption of ENGL1, however, did not lead to a phenotype distinct from the parental strain, indicating that this cell wall-associated beta(1-3)endoglucanase does not play an essential role in constitutive cell growth.
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pasteur-01710742 , version 1 (16-02-2018)

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Isabelle Mouyna, Jacqueline Sarfati, P. Recco, Thierry Fontaine, Bernard Henrissat, et al.. Molecular characterization of a cell wall-associated beta(1-3)endoglucanase of Aspergillus fumigatus.. Medical Mycology, 2002, 40 (5), pp.455-64. ⟨10.1080/mmy.40.5.455.464⟩. ⟨pasteur-01710742⟩
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