A fully synthetic immunogen carrying a carcinoma-associated carbohydrate for active specific immunotherapy. - Archive ouverte HAL Access content directly
Journal Articles Cancer Research Year : 1999

A fully synthetic immunogen carrying a carcinoma-associated carbohydrate for active specific immunotherapy.

(1) , (2) , (2) , (1) , (2) , (1)
1
2

Abstract

Aberrant glycosylation of mucins leads to the exposure of cryptic carbohydrate antigens at the surface of carcinoma cells, which, therefore, represent potent targets for anticancer therapeutic vaccines. To date, the development of immunogens to stimulate immune response to such saccharidic antigens is based on carbohydrate conjugation to carrier proteins. However, these traditional protein conjugates are poorly defined in chemical composition and structure. As an alternative, we synthesized a multiple antigenic O-linked glycopeptide (MAG) carrying the carbohydrate Tn antigen associated with a CD4+ T-cell epitope (MAG:Tn-PV). This fully synthetic immunogen is highly defined in composition and carries a high saccharidic epitope ratio over the entire molecule. The MAG:Tn-PV was able to induce anti-Tn IgG antibodies that recognize human tumor cell lines. A therapeutic immunization protocol performed with this fully synthetic immunogen increased the survival of tumor-bearing mice. Thus, the accurately defined and versatile MAG system represents an efficient strategy to induce carbohydrate-specific antitumor immune responses but may also be applicable to the prevention of infectious diseases, if it is based on bacterial oligosaccharides.
Not file

Dates and versions

pasteur-00166858 , version 1 (10-08-2007)

Identifiers

  • HAL Id : pasteur-00166858 , version 1
  • PUBMED : 10197623

Cite

R. Lo-Man, S. Bay, S. Vichier-Guerre, E. Dériaud, D. Cantacuzène, et al.. A fully synthetic immunogen carrying a carcinoma-associated carbohydrate for active specific immunotherapy.. Cancer Research, 1999, 59 (7), pp.1520-4. ⟨pasteur-00166858⟩

Collections

PASTEUR CNRS
24 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More