Terahertz biomems for enzymatic catalysis monitoring
Abstract
This work focuses on the fabrication and use of a Biological Micro-ElectroMechanical System (BioMEMS) for TeraHertz (THz) detection and characterization of enzymatic catalysis reactions. The fluidic microdevice was fabricated using traditional lithographic techniques. It was then functionalized by reactive amine functions using plasma polymerized allylamine (pp-allylamine), followed by enzyme immobilization inside the microchannels. The enzymatic reaction was controlled by fluorescent microscopy before being analysed by sub-THz spectroscopy in the frequency range 0.06–0.11 THz. The preliminary results show the feasibility of terahertz monitoring of the biocatalysis reaction.
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