Chapitre D'ouvrage Année : 2016

Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin

Résumé

The improvement of dendritic cell subset isolation from tissues and the use of appropriate surface markers allowed to decipher their heterogeneity but also allowed to unravel some specific functions that are valuable for vaccine design as well as for a better understanding of the in situ pathophysiology upon infection. Here, we describe the procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.

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Dates et versions

inserm-04871547 , version 1 (07-01-2025)

Identifiants

Citer

Camille Malosse, Sandrine Henri. Isolation of Mouse Dendritic Cell Subsets and Macrophages from the Skin. Dendritic Cell Protocols, 1423, Springer New York, pp.129-137, 2016, Methods in Molecular Biology, 978-1-4939-3604-5. ⟨10.1007/978-1-4939-3606-9_9⟩. ⟨inserm-04871547⟩
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