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Article Dans Une Revue Genesis Année : 2009

Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.

Résumé

To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders.

Dates et versions

inserm-00357169 , version 1 (29-01-2009)

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Olivia Wendling, Jean-Marc Bornert, Pierre Chambon, Daniel Metzger. Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.. Genesis, 2009, 47 (1), pp.14-8. ⟨10.1002/dvg.20448⟩. ⟨inserm-00357169⟩
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