Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Experimental Parasitology Année : 2011

Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.

Résumé

Detailed studies of host/parasite interactions are currently limited because in situ gene sequencing or monitoring of parasite gene expression is so far limited to genes presenting a high loci copy number in the Schistosome genome or a high level of expression. Indeed, how to investigate the host parasite molecular interplay when parasites are not directly accessible in vivo? Here we describe a method to circumvent this problem and to analyze DNA and RNA of Schistosoma mansoni during the interaction with its intermediate snail host Biomphalaria glabrata. We propose a technique for improved DNA and RNA extraction from the intra-molluscan stage of the parasite recovered after fixation of infected snails in Raillet-Henry solution. The extractions can be used for genetic analysis, transcription studies and microsatellite genotyping.
Fichier principal
Vignette du fichier
Tapuscrit-Allienne-2011-ExpPara.pdf (849.89 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

halsde-00612896 , version 1 (02-02-2012)

Identifiants

Citer

Jean-François Allienne, André Théron, Benjamin Gourbal. Recovery of primary sporocysts in vivo in the Schistosoma mansoni/Biomphalaria glabrata model using a simple fixation method suitable for extraction of genomic DNA and RNA.. Experimental Parasitology, 2011, 129 (1), pp.11-16. ⟨10.1016/j.exppara.2011.06.003⟩. ⟨halsde-00612896⟩
236 Consultations
249 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More