The Surduk loess section in Serbia provides a 20 m thick pedosedimentary record of the last interglacial–glacial climatic cycle (Upper Pleistocene). Based on optical dating, a chronostratigraphy could be established for the last climatic cycle, yielding the first numerical ages of a loess record from the middle Danube basin. Infrared-stimulated luminescence (IR-OSL) dating has been applied to the polymineral silt fraction using a multiple aliquot additive-dose protocol to determine the equivalent dose (DE). Within error limits, all age estimates are in stratigraphic order. Owing to the application of shine-plateau tests, the samples showed no evidence of insufficient bleaching. The Surduk loess section comprises three major periods of soil formation. Based on the IR-OSL chronostratigraphy, the lowermost pedocomplex is attributed to the Last Interglacial and to the Early Glacial (Marine Isotope Stage – MIS 5e to 5a). The middle part of the section exhibits a succession of weakly developed brown soils and a humic horizon, named 'Surduk soil', formed during MIS 3. On top of the section, recent soil formation is related to the Holocene. Thick loess deposits are preserved between these palaeosols and are attributed to the Lower and Upper Pleniglacials (MIS 4 and 2), respectively. Estimated mean sedimentation rates are 0.1–0.2 mm/yr for the last glacial cycle, with a strong increase to 0.6 mm/yr with onset of the Pleniglacial.