Article Dans Une Revue Microbiology Spectrum Année : 2026

Diversity and varying predation capacities of culturable Amoebozoae against opportunistic vibrios in contrasting Mediterranean coastal environments

Résumé

Free-living amoebae (FLA) are ubiquitous and can be found in many environments including soil, freshwater, and marine environments. They feed on various microorganisms and can play an important role in the food web and its dynamics. We previously described that FLA belonging to the Vannella genus isolated from oyster farms in the Thau lagoon in France are able to establish stable interactions with Vibrionaceae, suggesting they can play a role in pathogen dynamics. To further investigate the ecological interactions between FLA and Vibrionaceae in Mediterranean coastal waters, we conducted monthly sampling for 1 year at three contrasting sites. FLA populations were isolated from water and sediment samples on different bacterial lawns, including Escherichia coli or Vibrio tasmaniensis , Vibrio crassostreae , and Vibrio harveyi . Diversity analysis by v4-18S barcoding revealed distinct communities between fractions and sites. The Vannellidae were found significantly enriched in the water, whereas Paramoebidae were found enriched in the sediments. Additionally, uneven distribution of Vexilliferidae, Vermistellae, and, to a lesser extent, Acanthamoebidae and Subulatomonas contrasted between sampling sites. Selection of grazers on different bacterial lawns revealed that V. tasmaniensis inhibits the growth of most Vannellidae, whereas V. crassostreae inhibits the growth of Paramoebidae. These differences were further confirmed functionally using isolates belonging to each Amoebozoa taxonomic group. Overall, our results highlight the need for more comprehensive studies of the diversity and population dynamics of Amoebozoa in marine environments and indicate that the role of these diversified grazers in shaping Vibrio communities is complex and still poorly characterized.

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hal-05520580 , version 1 (20-02-2026)

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Étienne Robino, Angélique Perret, Cyril Noel, Philippe Haffner, Laurent Intertaglia, et al.. Diversity and varying predation capacities of culturable Amoebozoae against opportunistic vibrios in contrasting Mediterranean coastal environments. Microbiology Spectrum, inPress, ⟨10.1128/spectrum.01138-25⟩. ⟨hal-05520580⟩
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