<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-05468325</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-22T07:34:15+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">RNA polymerase II initiation factors show different dynamic behaviour upon induced transcription in live cells</title>
            <author role="aut">
              <persName>
                <forename type="first">Attlia</forename>
                <surname>Oravecz</surname>
              </persName>
              <email type="md5">d9986aa18457b455f0193d7a04f611c3</email>
              <email type="domain">unistra.fr</email>
              <idno type="idhal" notation="string">oravecz</idno>
              <idno type="idhal" notation="numeric">1485735</idno>
              <idno type="halauthorid" notation="string">3997588-1485735</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-7860-6606</idno>
              <idno type="IDREF">https://www.idref.fr/263257568</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Olivier</forename>
                <surname>Tassy</surname>
              </persName>
              <idno type="idhal" notation="numeric">1545285</idno>
              <idno type="halauthorid" notation="string">551470-1545285</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2088-155X</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sascha</forename>
                <surname>Conic</surname>
              </persName>
              <idno type="halauthorid">1585433-0</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Mustapha</forename>
                <surname>Oulad-Abdelghani</surname>
              </persName>
              <email type="md5">9f34dc1c24021b245411745515605558</email>
              <email type="domain">igbmc.fr</email>
              <idno type="idhal" notation="numeric">756886</idno>
              <idno type="halauthorid" notation="string">160027-756886</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-8381-4397</idno>
              <idno type="IDREF">https://www.idref.fr/149095066</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nacho</forename>
                <surname>Molina</surname>
              </persName>
              <email type="md5">13425d7f7fb0181f0c74abe70ce3f628</email>
              <email type="domain">igbmc.fr</email>
              <idno type="idhal" notation="string">nacho-molina</idno>
              <idno type="idhal" notation="numeric">180853</idno>
              <idno type="halauthorid" notation="string">46910-180853</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-0233-3055</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Laszlo</forename>
                <surname>Tora</surname>
              </persName>
              <email type="md5">fd4b7a88c2bae65c0a78be06086a3190</email>
              <email type="domain">igbmc.fr</email>
              <idno type="idhal" notation="string">laszlo-tora</idno>
              <idno type="idhal" notation="numeric">178874</idno>
              <idno type="halauthorid" notation="string">222043-178874</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/E-9999-2018</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-7398-2250</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=HXDPVH0AAAAJ&amp;hl=en</idno>
              <idno type="IDREF">https://www.idref.fr/069776113</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/E-9999-2018</idno>
              <affiliation ref="#struct-93800"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Laszlo</forename>
                <surname>Tora</surname>
              </persName>
              <email type="md5">fd4b7a88c2bae65c0a78be06086a3190</email>
              <email type="domain">igbmc.fr</email>
            </editor>
            <funder ref="#projanr-49561"/>
            <funder ref="#projanr-37796"/>
            <funder ref="#projanr-54655"/>
            <funder ref="#projanr-66690"/>
            <funder ref="#projanr-115654"/>
            <funder ref="#projeurop-121745"/>
            <funder>NIH MIRA (R35GM139564) and693</funder>
            <funder>National Science Foundation EFRI grant 193334</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2026-01-20 17:11:23</date>
              <date type="whenModified">2026-04-15 17:07:46</date>
              <date type="whenReleased">2026-01-23 07:56:11</date>
              <date type="whenProduced">2026-01-20</date>
              <date type="whenEndEmbargoed">2026-01-20</date>
              <ref type="file" target="https://hal.science/hal-05468325v1/document">
                <date notBefore="2026-01-20"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-05468325v1/file/2025.12.19.695093v1.full.pdf" id="file-5468325-4683998">
                <date notBefore="2026-01-20"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="377427">
                <persName>
                  <forename>Laszlo</forename>
                  <surname>Tora</surname>
                </persName>
                <email type="md5">fd4b7a88c2bae65c0a78be06086a3190</email>
                <email type="domain">igbmc.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-05468325</idno>
            <idno type="halUri">https://hal.science/hal-05468325</idno>
            <idno type="halBibtex">oravecz:hal-05468325</idno>
            <idno type="halRefHtml">2026</idno>
            <idno type="halRef">2026</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by-nc-nd/4.0/">CC BY-NC-ND 4.0 - Attribution - Non-commercial use - No Derivative Works<ref corresp="#file-5468325-4683998"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="IGBMC" corresp="INSERM">Institut Génétique de Biologie Moléculaire et Cellulaire</idno>
            <idno type="stamp" n="UNIV-STRASBG">Université de Strasbourg</idno>
            <idno type="stamp" n="OPENAIRE">OpenAIRE</idno>
            <idno type="stamp" n="SITE-ALSACE">Archive ouverte du site Alsace</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="UNIVOAK">UNIVOAK</idno>
            <idno type="stamp" n="UNISTRA-ITI">Publications ITI Strasbourg</idno>
          </seriesStmt>
          <notesStmt/>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">RNA polymerase II initiation factors show different dynamic behaviour upon induced transcription in live cells</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Attlia</forename>
                    <surname>Oravecz</surname>
                  </persName>
                  <email type="md5">d9986aa18457b455f0193d7a04f611c3</email>
                  <email type="domain">unistra.fr</email>
                  <idno type="idhal" notation="string">oravecz</idno>
                  <idno type="idhal" notation="numeric">1485735</idno>
                  <idno type="halauthorid" notation="string">3997588-1485735</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-7860-6606</idno>
                  <idno type="IDREF">https://www.idref.fr/263257568</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Olivier</forename>
                    <surname>Tassy</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1545285</idno>
                  <idno type="halauthorid" notation="string">551470-1545285</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2088-155X</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sascha</forename>
                    <surname>Conic</surname>
                  </persName>
                  <idno type="halauthorid">1585433-0</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Mustapha</forename>
                    <surname>Oulad-Abdelghani</surname>
                  </persName>
                  <email type="md5">9f34dc1c24021b245411745515605558</email>
                  <email type="domain">igbmc.fr</email>
                  <idno type="idhal" notation="numeric">756886</idno>
                  <idno type="halauthorid" notation="string">160027-756886</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-8381-4397</idno>
                  <idno type="IDREF">https://www.idref.fr/149095066</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nacho</forename>
                    <surname>Molina</surname>
                  </persName>
                  <email type="md5">13425d7f7fb0181f0c74abe70ce3f628</email>
                  <email type="domain">igbmc.fr</email>
                  <idno type="idhal" notation="string">nacho-molina</idno>
                  <idno type="idhal" notation="numeric">180853</idno>
                  <idno type="halauthorid" notation="string">46910-180853</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-0233-3055</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Laszlo</forename>
                    <surname>Tora</surname>
                  </persName>
                  <email type="md5">fd4b7a88c2bae65c0a78be06086a3190</email>
                  <email type="domain">igbmc.fr</email>
                  <idno type="idhal" notation="string">laszlo-tora</idno>
                  <idno type="idhal" notation="numeric">178874</idno>
                  <idno type="halauthorid" notation="string">222043-178874</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/E-9999-2018</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-7398-2250</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=HXDPVH0AAAAJ&amp;hl=en</idno>
                  <idno type="IDREF">https://www.idref.fr/069776113</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/E-9999-2018</idno>
                  <affiliation ref="#struct-93800"/>
                </author>
              </analytic>
              <monogr>
                <imprint>
                  <date type="datePub">2025-12-21</date>
                </imprint>
              </monogr>
              <idno type="biorxiv">2025.12.19.695093</idno>
              <idno type="doi">10.64898/2025.12.19.695093</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
              <classCode scheme="halOldTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
              <classCode scheme="halTreeTypology" n="UNDEFINED">Preprints, Working Papers, ...</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Abstract Transcription by RNA polymerase II (Pol II) requires the ordered action of general transcription factors (GTFs) forming the pre-initiation complex (PIC). How these events unfold kinetically remains unclear. Upon transcription activation, we observe coordinated recruitment of Pol II and GTFs together with chromatin decompaction. Pol II and its Ser5-phosphorylated form accumulate and persist at promoters, whereas TFIIB and TFIIF engage transiently, revealing distinct dynamic regimes. We find specific residence kinetics consistent with rapid exchange of GTFs and a more stable initiating Pol II population. A quantitative kinetic model recapitulates the temporal ordering and relative amplitudes of factor recruitment, predicts recruitment times, and links transient GTF engagement to sustained Pol II occupancy. Steady state measurements and transcription inhibition corroborate model predictions. These results uncover a dynamic hierarchy in human PIC assembly and establish a quantitative framework that connects factor exchange kinetics to the regulation of Pol II activity in living cells.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-93800" status="VALID">
          <idno type="IdRef">155263749</idno>
          <idno type="ISNI">0000 0004 0638 2716</idno>
          <idno type="RNSR">200112491C</idno>
          <idno type="ROR">https://ror.org/0015ws592</idno>
          <idno type="Wikidata">Q3152004</idno>
          <orgName>Institut de Génétique et de Biologie Moléculaire et Cellulaire</orgName>
          <orgName type="acronym">IGBMC</orgName>
          <date type="start">2009-01-01</date>
          <desc>
            <address>
              <addrLine>Parc D'Innovation 1 Rue Laurent Fries - BP 10142 67404 Illkirch Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.igbmc.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMRS964" active="#struct-199013" type="direct"/>
            <relation name="U1258" active="#struct-303623" type="direct"/>
            <relation name="UMR7104" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-199013" status="VALID">
          <idno type="IdRef">131056549</idno>
          <idno type="ROR">https://ror.org/00pg6eq24</idno>
          <orgName>Université de Strasbourg</orgName>
          <orgName type="acronym">UNISTRA</orgName>
          <desc>
            <address>
              <addrLine>4 rue Blaise Pascal - CS 90032 - 67081 Strasbourg cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.unistra.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-49561" status="VALID">
          <idno type="anr">ANR-17-EURE-0023</idno>
          <orgName>IMCBio</orgName>
          <desc>Integrative Molecular and Cellular Biology</desc>
          <date type="start">2017</date>
        </org>
        <org type="anrProject" xml:id="projanr-37796" status="VALID">
          <idno type="anr">ANR-10-IDEX-0002</idno>
          <idno type="program">Initiative d'excellence</idno>
          <orgName>UNISTRA</orgName>
          <desc>Par-delà les frontières, l'Université de Strasbourg</desc>
          <date type="start">2010</date>
        </org>
        <org type="anrProject" xml:id="projanr-54655" status="VALID">
          <idno type="anr">ANR-20-SFRI-0012</idno>
          <orgName>STRAT'US</orgName>
          <desc>Façonner les talents en formation et en recherche à l'Université de Strasbourg</desc>
          <date type="start">2020</date>
        </org>
        <org type="anrProject" xml:id="projanr-66690" status="VALID">
          <idno type="anr">ANR-22-CE11-0013</idno>
          <orgName>ATAC</orgName>
          <desc>Étude Structurale et Fonctionnelle du Complexe co-activateur ATAC humain</desc>
          <date type="start">2022</date>
        </org>
        <org type="anrProject" xml:id="projanr-115654" status="VALID">
          <idno type="anr">ANR-23-CE11-0034</idno>
          <orgName>ChromOrigami</orgName>
          <desc>Machines à base d'ADN Origami pour la manipulation épigénétique de la transcription des gènes</desc>
          <date type="start">2023</date>
        </org>
        <org type="europeanProject" xml:id="projeurop-121745" status="VALID">
          <idno type="number">340551</idno>
          <idno type="program">ERC-2013-ADG</idno>
          <idno type="call">ERC-2013-ADG</idno>
          <orgName>BIRTOACTION</orgName>
          <desc>From birth to action: regulation of gene expression through transcription complex biogenesis</desc>
          <date type="start">2014-01-01</date>
          <date type="end">2018-12-31</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>