Deciphering the intermolecular interactions between G-quadruplex (G4)-forming sequences
Résumé
Interactions between biomolecules govern cellular biology. While protein/protein and protein/nucleic acid (DNA, RNA) interactions-and, to a lesser extent, RNA/RNA and RNA/DNA interactions-ha v e been e xtensiv ely described, a question remains as to whether and how non-canonical DNA str uct ures might interact with each other. This is of particular interest for guanine (G)-rich sequences that can fold into G-quadruplex (G4) str uct ures: Individual G4s are currently studied for their involvement in a myriad of cellular events (mostly pertaining to the control of gene expression), and, more recently, the interactions between two G4s have been scrutinized as being part of a novel gene expression regulatory mechanism in v olving chromatin remodeling through G4-mediated loop f ormation. T he question that needs to be answered is whether G4s or their corresponding G-rich sequences are in v olv ed. We present here a series of results collected using a combination of sequences, experimental conditions, and tec hniques, whic h led us to the conclusion that G4/G4 intermolecular interactions are mostly go v erned b y primary sequence interactions in vitro.
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