Poster De Conférence Année : 2024

Functional characterization of a plastidial cytochrome b5-fused Δ4 desaturase from Ostreococcus tauri in higher plants

Résumé

Marine microalgae are the primary producers of important lipids in oceanic ecosystems. In particular, marine microalgae fuel the food web with omega-3 very-long chain polyunsaturated fatty acids (ω3-VLC-PUFAs), which provide a protective role against a range of human metabolic disorders and are therefore considered highly health beneficial. Ostreococcus tauri is a pico-eukaryote that contains 22:6 (DHA) in microsomal lipids as well as many ω3-PUFAs, including 18:5 and 16:4, which are highly abundant in plastidial galactolipids but also found in betaine and neutral lipids (Degraeve-Guilbault et al., 2017). The genome of O. tauri contains 13 genes putatively encoding fatty acid desaturases. In the present study, we characterized the enzyme encoded by OT_ostta13g01550 (CEG00114.1) as a plastidial cytochrome b5-fused delta-4 desaturase involved in 16:4 biosynthesis. Transient heterologous expression in Nicotiana benthamiana resulted in the production of 16:4Δ4,7,10,13 and 16:3Δ4,7,10, but failed to produce 18:5Δ3,6,9,12,15 when coexpressed with plastidial Δ6-desaturases. Stable transgenic Arabidopsis lines were also generated, and their characterization will be presented.

Fichier principal
Vignette du fichier
GERLI_abstract_F DOMERGUE.pdf (112.11 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

hal-05265564 , version 1 (17-09-2025)

Licence

Identifiants

  • HAL Id : hal-05265564 , version 1

Citer

Magdalena Miklaszewska, Rodrigo Enrique Gomez, Charlotte Degraeve-Guilbault, Pierre van Delft, Laetitia Fouillen, et al.. Functional characterization of a plastidial cytochrome b5-fused Δ4 desaturase from Ostreococcus tauri in higher plants. 19th GERLI International Lipidomics meeting, Sep 2024, Brest, France. ⟨hal-05265564⟩
152 Consultations
43 Téléchargements

Partager

  • More