Characterization of MdMYB68, a suberin master regulator in russeted apples
Résumé
Apple russeting is mainly due to the accumulation of suberin in the cell wall in response of defects and damages in the cuticle layer. Our previous studies highlighted several MYB transcription factors which might regulate the suberization in russeted or suberized apple fruit skin. Among these, we identified MdMYB68, which was co-expressed with number of well-known key suberin biosynthesis genes (Figure 1). Indeed, its transient expression in N. benthamiana triggered the expression of the whole suberin biosynthesis pathway (Figure 2). A lipid content analysis further confirmed that MdMYB68 regulates the deposition of suberin in cell walls (Figure 3 and Figure 4). Furthermore, we also investigated the alteration of the non-lipid cell wall components and showed that MdMYB68 trigger a massive modification of the hemicelluloses and pectins (Figure 5 and Figure 6).
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