Article Dans Une Revue UTTAR PRADESH JOURNAL OF ZOOLOGY Année : 2025

Qualitative and Molecular Characterization of Rugda Mushroom: A Comprehensive Analysis

Résumé

Rugda mushrooms are valued for their nutritional and therapeutic properties, yet detailed compositional and taxonomic studies remain scarce. In this work, we present an integrated quantitative analysis of phytochemicals and molecular characterization of Rugda fruiting bodies, employing Gas Chromatography–Mass Spectrometry (GC‑MS), Fourier‑Transform Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and DNA‑based phylogenetic analysis. GC‑MS analysis revealed nine compounds. Quantitative analysis further determined total phenolic content and total flavonoid content and the total phenolic content was 9.01±0.31 mg GAE⋅g−1 and total flavonoid content was 4.13 ± 0.12 mg QE·g⁻¹ observed. FTIR spectra exhibited characteristic peaks at O–H stretching, N-H bend, C–O strech, indicating phenol, amine & ester. SEM images showed a compact hyphal network with ellipsoid spores bearing reticulate ornamentation. ITS sequencing confirmed Rugda as belonging to the Astraeus asiaticus. The combined GC‑MS, FTIR, and SEM data elucidate the chemical and morphological attributes of Rugda mushroom, while ITS‑based molecular analysis provides accurate taxonomic placement. This multidisciplinary profiling lays a foundation for future exploration of Rugda’s bioactive compounds and supports its authentication for nutritional, pharmaceutical, and industrial applications.

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Dates et versions

hal-05211849 , version 1 (16-08-2025)

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  • HAL Id : hal-05211849 , version 1

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Neha Rai, Navita Gupta, Amar Kumar. Qualitative and Molecular Characterization of Rugda Mushroom: A Comprehensive Analysis. UTTAR PRADESH JOURNAL OF ZOOLOGY, 2025, 46 (15), pp.285-294. ⟨hal-05211849⟩
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