Poster De Conférence Année : 2025

Maximizing metabolome coverage and metabolism in Microcystis sp. : A comparative evaluation of monophasic and biphasic extraction-based workflow

Résumé

Cyanobacterial blooms are a phenomenon of growing international concern, due to their potential ecological, human and economic impacts. Recently, some studies have highlighted the potential role of metabolic interactions between heterotrophic bacteria and cyanobacteria in the phycosphere in promoting cyanobacterial blooms. In this context, there is a growing need to comprehensively depict metabolome and metabolism of cyanobacteria and heterotrophic bacteria consortium. Thus, this study aims to explore various metabolomic workflows in order to provide a comprehensive picture of the chemical landscape and so the metabolism for twelve strains of M. aeruginosa. Thus, our objective was to determine the complementarity and the specificity of these workflows in terms number of annotated metabolites and classes. To such an end, biphasic (MTBE/MeOH/HO leading to a hydrophilic and lipophilic extracts) and monophasic extraction (MeOH/HO leading to a single extract) coupled to UPLC-HRMS/MS analyses were compared. Biphasic extracts were separated according an hydrophilic and a lipophilic chromatographic gradient while monophasic extract was only separated on hydrophilic gradient prior data acquisition. Data were processed by combining MS-DIAL, MS-CleanR and SIRIUS. Monophasic extraction based-workflow highlighted marked differences in the chemical landscapes of the twelve cyanobacteria. In particular, some specialized metabolites (microcystins LA, LF) were specific to some strains. Data from biphasic workflow is under investigation. Overall, this work will support a better understanding of the role of metabolism and metabolic interaction in M. aeruginosa bloom formation.

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Dates et versions

hal-05173457 , version 1 (21-07-2025)

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  • HAL Id : hal-05173457 , version 1

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Thomas Forjonel, Mélissa Eon, Charlotte Duval, Sylvia Moreira, Benjamin Marie, et al.. Maximizing metabolome coverage and metabolism in Microcystis sp. : A comparative evaluation of monophasic and biphasic extraction-based workflow. RFMF, Jun 2025, Paris, France. 2025. ⟨hal-05173457⟩
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