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              <p>&lt;div&gt;&lt;p&gt;Bone xenografts represent a promising alternative to autologous or allograft transplants, yet antigenicity in animal-derived tissues remains a major limitation to their clinical use. To provide any risk of contamination or allogenic rejection, the Supercrit® process was developed to treat allogeneic human bone combining a supercritical CO 2 treatment followed by a chemical treatment using high quantities of different solvents. The aim of this study was to produce a xenogeneic bone substitute thanks to the development of a new one-step supercritical process, 'Goxcrit', and to test it in vivo. This new process reduces the use of solvents by injecting them under pressure into the supercritical CO 2 flow, while maintaining the cleaning quality of the bone matrix and better preserving its inner structure, essential for its future bone integration.&lt;/p&gt;&lt;p&gt;Porcine derived bone samples were treated using Goxcrit or Supercrit®, and compared with human bone treated with Supercrit®, the commercialized bone allograft. In vitro analyses demonstrated the absence of cytotoxicity and of the alpha-gal epitope mainly responsible for cross-species immunogenicity. Additionally, in vivo experiments revealed improved bone formation in rats critical calvarial defects (BV/TV and von Kossa analyses) implanted with Goxcrit samples, with bone remodeling (TRAP/ALP stains), compared to those treated with Supercrit®. These results can be attributed to the less aggressive chemical process of the Goxcrit, which preserves the bone's inner structure critical for remodeling.&lt;/p&gt;&lt;p&gt;Our study highlighted the interest of using a porcine bone source treated with the Goxcrit process to meet the growing demand for reliable and effective bone substitutes.&lt;/p&gt;&lt;/div&gt;</p>
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