<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-05147082</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-15T19:11:43+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Determination of the Lethal Dose of Ethyl Methane Sulfonate (EMS) for Optimal In-vitro Mutagenesis in Banana (Musa spp. cv. Phee-gyan)</title>
            <author role="aut">
              <persName>
                <forename type="first">Myo Myat Myat</forename>
                <surname>Kyaw</surname>
              </persName>
              <idno type="halauthorid">3627898-0</idno>
              <affiliation ref="#struct-1373377"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Phyu Phyu</forename>
                <surname>Oo</surname>
              </persName>
              <idno type="halauthorid">3627899-0</idno>
              <affiliation ref="#struct-1373377"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Aye Thet</forename>
                <surname>Hnin</surname>
              </persName>
              <idno type="halauthorid">3627900-0</idno>
              <affiliation ref="#struct-1373379"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Moe Kyaw</forename>
                <surname>Thu</surname>
              </persName>
              <idno type="halauthorid">728057-0</idno>
              <affiliation ref="#struct-1373380"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Myint</forename>
                <surname>Aye</surname>
              </persName>
              <idno type="idhal" notation="numeric">1559476</idno>
              <idno type="halauthorid" notation="string">3627901-1559476</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0466-1805</idno>
              <affiliation ref="#struct-1373377"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Aye Nyein</forename>
                <surname>Chan</surname>
              </persName>
              <idno type="idhal" notation="numeric">1559477</idno>
              <idno type="halauthorid" notation="string">3627902-1559477</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-4248-1538</idno>
              <affiliation ref="#struct-1373377"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Mind</forename>
                <surname>Canvas</surname>
              </persName>
              <email type="md5">0ee776b7d8905f5742c2d8a2da75a43c</email>
              <email type="domain">gmail.com</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2025-07-07 07:24:27</date>
              <date type="whenModified">2026-02-13 15:12:14</date>
              <date type="whenReleased">2025-07-07 07:24:27</date>
              <date type="whenProduced">2025-07-05</date>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="1670012">
                <persName>
                  <forename>Mind</forename>
                  <surname>Canvas</surname>
                </persName>
                <email type="md5">0ee776b7d8905f5742c2d8a2da75a43c</email>
                <email type="domain">gmail.com</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-05147082</idno>
            <idno type="halUri">https://hal.science/hal-05147082</idno>
            <idno type="halBibtex">kyaw:hal-05147082</idno>
            <idno type="halRefHtml">&lt;i&gt;Asian Plant Research Journal&lt;/i&gt;, 2025, 13 (3), pp.87-101</idno>
            <idno type="halRef">Asian Plant Research Journal, 2025, 13 (3), pp.87-101</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt/>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Determination of the Lethal Dose of Ethyl Methane Sulfonate (EMS) for Optimal In-vitro Mutagenesis in Banana (Musa spp. cv. Phee-gyan)</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Myo Myat Myat</forename>
                    <surname>Kyaw</surname>
                  </persName>
                  <idno type="halauthorid">3627898-0</idno>
                  <affiliation ref="#struct-1373377"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Phyu Phyu</forename>
                    <surname>Oo</surname>
                  </persName>
                  <idno type="halauthorid">3627899-0</idno>
                  <affiliation ref="#struct-1373377"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Aye Thet</forename>
                    <surname>Hnin</surname>
                  </persName>
                  <idno type="halauthorid">3627900-0</idno>
                  <affiliation ref="#struct-1373379"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Moe Kyaw</forename>
                    <surname>Thu</surname>
                  </persName>
                  <idno type="halauthorid">728057-0</idno>
                  <affiliation ref="#struct-1373380"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Myint</forename>
                    <surname>Aye</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1559476</idno>
                  <idno type="halauthorid" notation="string">3627901-1559476</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0466-1805</idno>
                  <affiliation ref="#struct-1373377"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Aye Nyein</forename>
                    <surname>Chan</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">1559477</idno>
                  <idno type="halauthorid" notation="string">3627902-1559477</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-4248-1538</idno>
                  <affiliation ref="#struct-1373377"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">158749</idno>
                <idno type="issn">2581-9992</idno>
                <title level="j">Asian Plant Research Journal</title>
                <imprint>
                  <publisher>Ms. M. B. Mondal, Ph.D.</publisher>
                  <biblScope unit="volume">13</biblScope>
                  <biblScope unit="issue">3</biblScope>
                  <biblScope unit="pp">87-101</biblScope>
                  <date type="datePub">2025-07-05</date>
                </imprint>
              </monogr>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.sa">Life Sciences [q-bio]/Agricultural sciences</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>The genetic variation in Musa (ABB group, Bluggoe subgroup) cv. ‘Phee-gyan’, a popular cultivar in Myanmar, was enhanced by in vitro application of ethyl methane sulphonate (EMS) mutagenesis to proliferated shoot tips. Shoot tips were treated with EMS at concentrations of 0%, 0.3%, 0.6%, 0.9%, 1.2%, and 1.5% across three incubation periods (30 min, 60 min, and 90 min) and cultured in five replicates using a randomized block design. The experiment was conducted at the laboratories of New Genetics (YAU) and Biotechnology Research Section (DAR) during 2023-2024. The results showed a dose-dependent decrease in survival percentage, shoot length, and fresh weight in the M1V1 generation compared to the control. The highest survival rate (91.48%) was recorded at 0.3% EMS (30 min), while the lowest survival (31.29%) occurred at 1.5% EMS (90 min). A 50% survival rate was recorded at 1.2% EMS (90 min), indicating a balance between mutation induction and plant viability. Shoot length was reduced to ~ 50% of the control at 1.2% EMS across all incubation times, while fresh weight decreased to ~ 50% of the control at 1.5% EMS (60 min and 90 min). Determining the lethal dose (LD₅₀) is essential to identify the optimum EMS concentration that maximizes mutagenesis efficiency while minimizing plant damage. Probit analysis determined LD₅₀ values based on survival percentage as 1.9% EMS (30 min and 60 min), and 1.2% EMS (90 min). For shoot length, LD₅₀ values were 1.3% EMS (30 min), and 1.1% EMS (60 min and 90 min), while for fresh weight, they were 1.9% EMS (30 min), 1.7% EMS (60 min), and 1.5% EMS (90 min). Based on these findings, 1.2% EMS (90 min) was determined to be the optimal mutagenesis condition for inducing genetic variability in banana cv. ‘Phee-gyan’. This optimized treatment could facilitate the improvement of key traits in banana breeding programs.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-1373377" status="INCOMING">
          <orgName>Department of New Genetics, Advanced Centre for Agricultural Research and Education (ACARE), Yezin Agricultural University (YAU), Nay Pyi Taw, Myanmar.</orgName>
          <desc>
            <address>
              <country key="MM"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-1373379" status="INCOMING">
          <orgName>Department of Plant Pathology, Yezin Agricultural University (YAU), Nay Pyi Taw, Myanmar.</orgName>
          <desc>
            <address>
              <country key="MM"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-1373380" status="INCOMING">
          <orgName>Department of Agricultural Biotechnology, Yezin Agricultural University (YAU), Nay Pyi Taw, Myanmar.</orgName>
          <desc>
            <address>
              <country key="MM"/>
            </address>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>