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              <p>Antibiotic resistance (ABR) is a major global public health issue, recognized as an urgent priority by international institutions. Especially regarding the emergence and the global dissemination of multidrug-resistant bacteria (MDRB) and antibiotic resistance genes (ARGs), as they can spread widely across humans, animals and environmental domains.ABRomics-analysis is an online platform designed for tracking MDRB and ARGs from bacterial genomes and metagenomes. This platform aims to provide a tool for the surveillance and research in antimicrobial resistance in a One Health context. While ABRomics currently supports only genomic analyses, focusing on isolated bacterial genomes obtained through culture-based methods, the development of metagenomic workflows is underway.Metagenomics enables the direct analysis of all genetic material within a sample, bypassing the need for bacterial isolation. To harness this potential, we are developing three Galaxy workflows designed to extract different levels of information from metagenomic samples.The first workflow processes raw sequencing reads to generate an initial characterization of the sample’s resistome and taxonomic composition. It provides a rapid assessment of the antimicrobial resistance genes (ARGs) and microbial communities present in the sample.The second workflow focuses on gene-level analysis by assembling raw reads into contigs, enabling a more refined exploration of the resistome, taxonomic diversity, functional potential, and mobile genetic elements. This approach allows for a better understanding of the genetic context of resistance genes.The third workflow reconstructs Metagenome-Assembled Genomes (MAG), providing a genome-resolved view of the microbial community. Each MAG is classified by taxonomy and its resistome, mobilome, and functional annotations are determined.Together, these workflows aim to extend the functionalities of ABRomics beyond genomic surveillance, providing a comprehensive framework for the metagenomic surveillance of antimicrobial resistance.ABRomics-analysis is accessible at https://analysis.abromics.fr/</p>
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